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| Line 16: | Line 16: | ||
* [[Strain freezing]] | * [[Strain freezing]] | ||
* [[Solution Recipes]] | * [[Solution Recipes]] | ||
* Male generation and maintenance | |||
* Crossing to High Incidence of Males (him-5, him-8) strains | * Crossing to High Incidence of Males (him-5, him-8) strains | ||
| Line 26: | Line 27: | ||
* MiniMos | * MiniMos | ||
* Irradiation to produce integrant strains (IS) | * Irradiation to produce integrant strains (IS) | ||
* | |||
==== Cloning ==== | |||
* Restriction Digestion | |||
* Gibson Assembly | |||
* Gel Extraction / purification | |||
* Transformation | |||
* Plasmid Purification | |||
== Onboarding Materials == | == Onboarding Materials == | ||
Revision as of 16:15, 29 May 2024
Welcome to the Yemini Laboratory's Internal Wiki!
This resource is designed to provide current and accurate information about the equipment, protocols, and tools used in our lab. We hope that you will find it to be a valuable tool for staying informed and up-to-date on the work being done here.
If you have any questions or suggestions for improvement, please feel free to contact us. Thanks for visiting!
Material Safety Data Sheets (MSDS)
Protocols
General Worm Husbandry
- Strain cleaning
- Strain freezing
- Solution Recipes
- Male generation and maintenance
- Crossing to High Incidence of Males (him-5, him-8) strains
Behavioral Protocols
Generating Transgenics
- Microinjection of young adult worm gonad
- MiniMos
- Irradiation to produce integrant strains (IS)
Cloning
- Restriction Digestion
- Gibson Assembly
- Gel Extraction / purification
- Transformation
- Plasmid Purification