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Welcome to the Yemini Laboratory's Internal Wiki!

This resource is designed to provide current and accurate information about the equipment, protocols, and tools used in our lab.

We hope that you will find it to be a valuable tool for staying informed and up-to-date on the work being done here.

If you have any questions or suggestions for improvement, please feel free to contact us. Thanks for visiting!

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Material Safety Data Sheets (MSDS)

Lab Jobs

Oligo Ordering

Protocols

General Worm Husbandry

• Strain cleaning
• Strain freezing
• Solution Recipes
• Male generation and maintenance
• Crossing to High Incidence of Males (him-5, him-8) strains

Behavioral Protocols

• Drop Testing
• Mating Behavior Assays

Generating Transgenics

• Microinjection of young adult worm gonad
• MiniMos
• Irradiation to produce integrant strains (IS)

Cloning

• Competent cell transformation
• Restriction Digestion
• Gibson Assembly
• Gel Extraction / purification
• Transformation
• Plasmid Purification

Logistics

• Ordering cheat sheet
• Shipping with eShipGlobal
• Fedex shipping [DEPRECIATED]

General-use hardware and software

• Basler ace-2 aca4024-29um

Onboarding Materials

• Onboarding Steps

NeuroPAL_ID Software

• Developer Documentation
• Compiling NeuroPAL ID
• NeuroPAL ID Profiles
• Versioning and AppDesigner
• List of known NeuroPAL_ID Bugs