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(added Fedex shipping protocol under Logistics) |
(→Logistics: added ordering cheat sheet) |
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[[Lab Jobs]] | [[Lab Jobs]] | ||
[[Oligo Ordering]] | |||
== Protocols == | == Protocols == | ||
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==== Cloning ==== | ==== Cloning ==== | ||
* [[Competent cell transformation]] | |||
* Restriction Digestion | * Restriction Digestion | ||
* Gibson Assembly | * Gibson Assembly | ||
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==== Logistics ==== | ==== Logistics ==== | ||
* [[Fedex shipping]] | * [[Ordering cheat sheet]] | ||
* [[Shipping|Shipping with eShipGlobal]] | |||
* [[Fedex shipping]] [DEPRECIATED] | |||
== General-use hardware and software == | == General-use hardware and software == | ||
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== NeuroPAL_ID Software == | == NeuroPAL_ID Software == | ||
* [https://yeminilab.a2hosted.com/npal/docs/dev/_html/index.html Developer Documentation] | |||
* [[Compiling NeuroPAL ID]] | * [[Compiling NeuroPAL ID]] | ||
* [[NeuroPAL ID Profiles]] | * [[NeuroPAL ID Profiles]] | ||
* [[Versioning and AppDesigner]] | * [[Versioning and AppDesigner]] | ||
* [[NeuroPAL ID Bugs|List of known NeuroPAL_ID Bugs]] | * [[NeuroPAL ID Bugs|List of known NeuroPAL_ID Bugs]] | ||
Latest revision as of 16:36, 26 November 2025
Welcome to the Yemini Laboratory's Internal Wiki!
This resource is designed to provide current and accurate information about the equipment, protocols, and tools used in our lab.
We hope that you will find it to be a valuable tool for staying informed and up-to-date on the work being done here.
If you have any questions or suggestions for improvement, please feel free to contact us. Thanks for visiting!
Material Safety Data Sheets (MSDS)
Protocols
General Worm Husbandry
- Strain cleaning
- Strain freezing
- Solution Recipes
- Male generation and maintenance
- Crossing to High Incidence of Males (him-5, him-8) strains
Behavioral Protocols
Generating Transgenics
- Microinjection of young adult worm gonad
- MiniMos
- Irradiation to produce integrant strains (IS)
Cloning
- Competent cell transformation
- Restriction Digestion
- Gibson Assembly
- Gel Extraction / purification
- Transformation
- Plasmid Purification
Logistics
- Ordering cheat sheet
- Shipping with eShipGlobal
- Fedex shipping [DEPRECIATED]